AP21441SU-N Chicken IgA+IgG+IgM (H+L chain) antibody

Search for all "Chicken IgA+IgG+IgM"

1 ml / €250.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP21441SU-N

Product Description for Chicken IgA+IgG+IgM

Properties: (H+L chain)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Chicken IgA+IgG+IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Chicken IgA, Chicken IgG, Chicken IgM
Presentation Serum
Reactivity Chk
Applications ID, IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified normal Ig fractions isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other dog serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Precipitating techniques as Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal Chicken serum.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody Titre: Precipitin titre not less than 1/64 when tested against normal Chicken serum in agar block titration.
Concentration Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Format
State:
Lyophilized, Delipidated, Heat inactivated, Stable Whole Antiserum without preservatives
Serum
Reconstitution:
Restore by adding 1 ml of sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins.
It does not react with any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Species:
Human.
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