R1300AP Chicken IgG / Chicken IgY (H+L chain) antibody

Search for all "Chicken IgG / Chicken IgY"

1 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1300AP

Product Description for Chicken IgG / Chicken IgY

Properties: (H+L chain)
Presentation: AP

Properties for Chicken IgG / Chicken IgY

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Chicken Immunoglobulin G, Chicken Immunoglobulin Y
Presentation AP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Chicken IgG whole molecule.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product, in Western Blot 1:1,000, Immunohistochemistry 1:500.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Liquid (sterile filtered) purified Ig fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG and Chicken Serum.
  • LinkedIn