R1300B Chicken IgG / Chicken IgY (H+L chain) antibody

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1.5 mg / €250.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1300B

Product Description for Chicken IgG / Chicken IgY

Properties: (H+L chain)
Presentation: Biotin

Properties for Chicken IgG / Chicken IgY

Product Category Secondary Antibodies
Target Category
Quantity 1.5 mg
Synonyms Chicken Immunoglobulin G, Chicken Immunoglobulin Y
Presentation Biotin
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Chicken IgG whole molecule.
Application Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Concentration 1.5 mg/ml (by UV absorbance at 280 nm, before lyophil.)
Product Citations
Purchased from Acris:
  1. Martínez-Vega R, Garrido F, Partearroyo T, Cediel R, Zeisel SH, Martínez-Álvarez C, et al. Folic acid deficiency induces premature hearing loss through mechanisms involving cochlear oxidative stress and impairment of homocysteine metabolism. FASEB J. 2015 Feb;29(2):418-32. doi: 10.1096/fj.14-259283. Epub 2014 Nov 10. PubMed PMID: 25384423.
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule.
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against Chicken immunoglobulins.
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