SM2273P Chicken IgG / Chicken IgY (heavy chain) antibody

Search for all "Chicken IgG / Chicken IgY"

0.25 mg / €480.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

SM2273P

Product Description for Chicken IgG / Chicken IgY

Properties: (heavy chain)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for Chicken IgG / Chicken IgY

Product Category Secondary Antibodies
Target Category
Quantity 0.25 mg
Synonyms Chicken Immunoglobulin G, Chicken Immunoglobulin Y
Presentation Purified
Reactivity Duck
Applications E, F, WB
Clonality Monoclonal
Clone 16C7
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Isotype control AM03095PU-N
Application Flow Cytometry.
ELISA.
Western Blot under non-reducing conditions.
Background The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab)2 fragment of IgY.
Concentration 0.5 mg/ml
General Readings
  1. Magor KE, Higgins DA, Middleton DL, Warr GW. One gene encodes the heavy chains for three different forms of IgY in the duck. J Immunol. 1994 Dec 15;153(12):5549-55. PubMed PMID: 7989756.
  2. Higgins DA, Warr GW. Duck immunoglobulins: structure, functions and molecular genetics. Avian Pathol. 1993 Jun;22(2):211-36. PubMed PMID: 18671013.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS containing 0.09% Sodium Azide
State:
Liquid purified IgG
Purified
Specificity
Specificity:
This antibody recognises both the IgY and IgY(-Fc) heavy chain molecules. This antibody also detects IgY heavy chain in Mallard ducks, goose and swan. This antibody detects bands of approximately 118kDa and 178-200kDa in Pekin duck cell lysates under nonreducing conditions in Western blotting.
Species:
Duck. Does not react with Chicken.
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