discontinued

NB120-5753 Goat IgG (H and L) - F(ab)2 Fragment antibody

Search for all "Goat IgG (H and L) - F(ab)2 Fragment"

0.25 mg / €250.00

NB120-5753

Product Description for Goat IgG (H and L) - F(ab)2 Fragment

Presentation: Biotin
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Goat IgG (H and L) - F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation Biotin
Reactivity Gt
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Goat IgG whole molecule
Add. information Dot: Use at a dilution of 1/10000. ELISA: Use at a dilution of 1/40000 - 1/200000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. FACS: Use at a dilution of 1/200 - 1/1000. IHC: Use at a dilution of 1/500 - 1/5000. IM: Use at an assay dependent dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, flow cytometry / FACS analysis, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at a dilution of 1/10000. ELISA: Use at a dilution of 1/40000 - 1/200000. This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. FACS: Use at a dilution of 1/200 - 1/1000. IHC: Use at a dilution of 1/500 - 1/5000. IM: Use at an assay dependent dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., flow cytometry / FACS analysis 1:200 to 1:1000, ELISA, immunohistochemistry 1:500 to 1:5000, immunofluorescence, Western Blot 1:10000
Concentration 1 mg/ml
General Readings General / background references:Bayer EA & Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990). PubMed PMID: 2388567
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
Biotin
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human Serum Proteins.
Species:
Goat
  • LinkedIn