discontinued

NB120-5755 Goat IgG (H and L) - F(ab)2 Fragment antibody

Search for all "Goat IgG (H and L) - F(ab)2 Fragment"

0.25 mg / €250.00

NB120-5755

Product Description for Goat IgG (H and L) - F(ab)2 Fragment

Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Goat IgG (H and L) - F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation HRP
Reactivity Gt
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Goat IgG whole molecule.
Add. information This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Application ELISA, Western Blot, This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity., ELISA 1/10000 - 1/50000, Western Blot 1/5000
Concentration 1 mg/ml
General Readings General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
Affinity purified
Buffer System:
0.01% Gentamicin Sulfate
Purity:
Affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Species:
Goat
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