discontinued

NB120-6667 Goat IgG (H and L) - F(ab) Fragment antibody

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0.5 mg / €270.00

NB120-6667

Product Description for Goat IgG (H and L) - F(ab) Fragment

Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Goat IgG (H and L) - F(ab) Fragment

Product Category Secondary Antibodies
Quantity 0.5 mg
Presentation HRP
Reactivity Gt
Applications E, P, WB
Clonality Polyclonal
Host Donkey
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Goat IgG whole molecule
Add. information This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/3000 to 1/13000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Application Dot Blot, ELISA, immunohistochemistry, Immunomicroscopy, Western Blot, This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/3000 to 1/13000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity., ELISA 1:3000 - 1:13000, immunohistochemistry , Western Blot , Dot Blot , Immunomicroscopy
Concentration 1 mg/ml
General Readings General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
Affinity purified
Buffer System:
0.01% Gentamicin Sulfate
Purity:
Affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
Species:
Goat
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