R1416R Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

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0.5 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1416R

Product Description for Goat IgG

Properties: (H+L chain), F(ab)2 Fragment, adsorbed
Presentation: PE

Properties for Goat IgG

Product Category Secondary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Goat Immunoglobulin G
Presentation PE
Clonality Polyclonal
Host Donkey
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Goat IgG whole molecule
Application Suitable for Immunomicroscopy (1/100-1/200) and Flow Cytometry (1/100-1/200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Concentration 0.65 mg/ml (determined by absorbance at 565 nm for 0.1% Solution)
General Readings
  1. Carlsson J, Drevin H, Axén R. Protein thiolation and reversible protein-protein conjugation. N-Succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. Biochem J. 1978 Sep 1;173(3):723-37. PubMed PMID: 708370. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C prior to opening.
DO NOT FREEZE!
Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
R-Phycoerythrin (( from seaweed; Molecular Weight 240,000 daltons). Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound)
PE
Absorption emission:
488/575 nm
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and protease free), 0.05% (w/v) Sodium Azide as preservative.
Purity:
/
State:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1 ml distilled sterile water.
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum.
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Syrian Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins or other Goat heavy or light chain proteins.
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