AP33060HR-N Guinea Pig IgG (Fc specific) antibody

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1 ml / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Guinea Pig IgG

Properties: (Fc specific)
Presentation: HRP
Product is tested for Western blot / Immunoblot, Dot blot, Paraffin Sections, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay.

Properties for Guinea Pig IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Guinea Pig Immunoglobulin G
Presentation HRP
Reactivity GP
Applications Dot, E, ICC/IF, P, WB
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified normal IgG isolated from pooled guinea pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate technique for the binding to peroxidase, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life.
Application ELISA.
Dot blot. 

Immunohistochemistry on Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Horseradish peroxidase enriched for isoenzyme C (RZ=3.2)
Molar radio:
Peroxidase/IgG protein ~1.1
Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2
None, as it may interfere with the antibody activity
Lyophilized purified hyperimmune IgG fraction
Restore by adding 1 ml of sterile distilled water
The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity:  Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Guinea Pig.
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