R1322F Guinea pig IgG (H+L chain) antibody

Search for all "Guinea pig IgG"

2 mg / €220.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1322F

Product Description for Guinea pig IgG

Properties: (H+L chain)
Presentation: FITC

Properties for Guinea pig IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Guinea pig Immunoglobulin G
Presentation FITC
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Guinea Pig IgG whole molecule.
Application This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Concentration 2.2 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Blakeslee & Baines, J. Immunol. Methods 13; 305-320, 1976. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Molecular Weight 390 daltons
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
2.0 moles DTAF per mole of Rabbit IgG
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.01M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Thimerisol as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum.
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