AP33061SU-N Guinea Pig IgG1 (subclass specific) antibody

Search for all "Guinea Pig IgG1"

1 ml / €250.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP33061SU-N

Product Description for Guinea Pig IgG1

Properties: (subclass specific)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Guinea Pig IgG1

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Guinea Pig Immunoglobulin G1
Presentation Serum
Reactivity GP
Applications ID, IP
Clonality Polyclonal
Host Sheep
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Highly purified normal IgG1 isolated from pooled guinea pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Immunoprecipitation.
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in guinea pig serum and other body fluids or to determine its concentration.
Directions for Use:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/16 when tested against normal guinea pig serum in agar block titration.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
State:
Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
Serum
Reconstitution:
Restore by adding 1 ml of sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc subunit of subclass IgG1. It does not react with other IgG2, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.
Species:
Guinea Pig.
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