R1327B Hamster IgG (H+L chain) antibody

Search for all "Hamster IgG"

1.5 mg / €240.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Hamster IgG

Properties: (H+L chain)
Presentation: Biotin

Properties for Hamster IgG

Product Category Secondary Antibodies
Target Category
Quantity 1.5 mg
Synonyms Hamster Immunoglobulin G
Presentation Biotin
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Hamster IgG whole molecule
Application Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0µg of hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested for this product.
Concentration 1.5 mg/ml (OD 280 nm)
General Readings Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Shelf life: one year from despatch.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Immunoaffinity chromatography using Hamster IgG coupled to agarose
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
Lyophilized IgG fraction
Restore with 1.0 ml of deionized water or equivalent.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
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