R1327B Hamster IgG (H+L chain) antibody

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1.5 mg / €240.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1327B

Product Description for Hamster IgG

Properties: (H+L chain)
Presentation: Biotin

Properties for Hamster IgG

Product Category Secondary Antibodies
Target Category
Quantity 1.5 mg
Synonyms Hamster Immunoglobulin G
Presentation Biotin
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Hamster IgG whole molecule
Application Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0µg of hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested for this product.
Concentration 1.5 mg/ml (OD 280 nm)
General Readings Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Rabbit IgG molecule
Purification:
Immunoaffinity chromatography using Hamster IgG coupled to agarose
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide
State:
Lyophilized IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
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