R1327F Hamster IgG (H+L chain) antibody

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1.5 mg / €220.00
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R1327F

Product Description for Hamster IgG

Properties: (H+L chain)
Presentation: FITC

Properties for Hamster IgG

Product Category Secondary Antibodies
Target Category
Quantity 1.5 mg
Synonyms Hamster Immunoglobulin G
Presentation FITC
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Hamster IgG whole molecule.
Application Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
Flow Cytometry: 1/2,000-1/10,000.
IF Microscopy: 1/500-1/2,500.
Concentration 1.0 mg/ml
General Readings
  1. Blakeslee & Baines, J. Immunol. Methods 13; 305-320, 1976. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Flourescence isothiocyanate , Mol Weight 390 daltons
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
4.5 moles FITC per mole of Rabbit IgG
Purification:
Immunoaffinity Chromatography using Hamster IgG coupled to agarose.
Buffer System:
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium azide as preservative.
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.5 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
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