R1343HRP Human IgA + IgG + IgM antibody

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2 mg / €280.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1343HRP

Product Description for Human IgA + IgG + IgM

Presentation: HRP

Properties for Human IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 2 mg
Synonyms Human IgA, Human IgG, Human IgM
Presentation HRP
Reactivity Hu
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human IgG, IgA and IgM whole molecules in goat.
Application Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA 1/20,000-1/100,000.
Western blot 1/2,000-1/10,000.
Immunoprecipitation: 1/1,000-1/5,000.
Caution Do Not Add Sodium Azide.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. von Rhein C, Hunfeld KP, Ludwig A. Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection. Infect Immun. 2006 Nov;74(11):6505-8. Epub 2006 Aug 21. PubMed PMID: 16923786. (Free PMC Article available, 1 images available)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as a preservative.
Purity:
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a si
State:
Lyophilized purified Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum, Human IgG, Human IgA and Human IgM.
This reagent is suitable for the detection of all Human immunoglobulin isotypes and chain combinations.
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