R1399AP Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) antibody

Search for all "Human IgA + IgG + IgM"

0.5 mg / €310.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1399AP

Product Description for Human IgA + IgG + IgM

Properties: (adsorbed F(ab)2 Fragment)
Presentation: AP

Properties for Human IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 0.5 mg
Synonyms Human IgA, Human IgG, Human IgM
Presentation AP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human IgG whole molecule
Application Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Concentration 0.5 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
Storage This antibody is stable at 2-8°C as an undiluted liquid.
DO NOT FREEZE!
Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography
Buffer System:
0.01M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
State:
Liquid (sterile filtered) F(ab')2 fragments
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum.
This reagent is suitable for the detection of all human immunoglobulins and chain combinations.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Mouse Serum Proteins.
  • LinkedIn