R1399B Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) antibody

Search for all "Human IgA + IgG + IgM"

1 mg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Human IgA + IgG + IgM

Properties: (adsorbed F(ab)2 Fragment)
Presentation: Biotin

Properties for Human IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Human IgA, Human IgG, Human IgM
Presentation Biotin
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Human IgG, IgA and IgM whole molecules
Application ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (Cat.-No RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested for this product.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at RT.
This product is stable for one month at 2-8°C as an undiluted liquid.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio:
10-20 BAC molecules per Goat IgG molecule
Prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Buffer System:
0.02M Potassium Phosphate, 0.15 MSodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Lyophilized F(ab')2 fragments
Restore with 1.0 ml of deionized water (or equivalent).
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. No reaction was observed against Mouse Serum proteins.
This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
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