R1399HRP Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) antibody

Search for all "Human IgA + IgG + IgM"

0.5 mg / €270.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1399HRP

Product Description for Human IgA + IgG + IgM

Properties: (adsorbed F(ab)2 Fragment)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Human IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 0.5 mg
Synonyms Human IgA, Human IgG, Human IgM
Presentation HRP
Applications C, E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human IgG, IgA, and IgM whole molecules
Application Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product.
Caution Do NOT add Sodium Azide!
Concentration 0.5 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin Sulfate
Stabilizers:
10 mg/ml BSA (Ig and Protease free)
State:
Lyophilized F(ab')2 fragments
Reconstitution:
Restore with 0.5 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
No reaction was observed against anti-Goat IgG F(c) or Mouse Serum Proteins.
This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
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