DDX0290P-100 Human IgE antibody

Search for all "Human IgE"

0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Human IgE

Presentation: Purified
Product is tested for Western blot / Immunoblot, Functional assay, Flow Cytometry.

Properties for Human IgE

Product Category Secondary Antibodies
Quantity 0.1 mg
Synonyms Immunoglobulin E human
Presentation Purified
Reactivity Hu
Applications F, FN, WB
Clonality Monoclonal
Clone I27/mAb27
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Immunogen:
Purified human IgE (preincubated with mouse serum anti human IgG)
Application FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). 
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Cell surface receptors for the Fc fragments of IgE (FcR) have been identified on a variety of cell types from hematopoietic origin. The low affinity receptor for IgE (CD23) has been described as a B cell-activation marker. IgE-binding factors have been shown to play a major role in the regulation of IgE production. Basophils and mast cells express FcR1, which bind IgE with high affinity and the reaction of allergen with cell-bound IgE induces the release of a variety of pharmacologically active mediators that are responsible for the clinical manifestations of allergic disorders. mAb27 was generated from a mouse immunization program aiming at producing mAb specific for human IgE-Fc in order to map the sites on IgE interacting with receptors on target cells. (Chrétien I. et al, J. Immunol, 1988, (9) 141, pp3128-34).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Chrétien I, Helm BA, Marsh PJ, Padlan EA, Wijdenes J, Banchereau J. A monoclonal anti-IgE antibody against an epitope (amino acids 367-376) in the CH3 domain inhibits IgE binding to the low affinity IgE receptor (CD23). J Immunol. 1988 Nov 1;141(9):3128-34. PubMed PMID: 2459242.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Buffer System:
100 μg in 200 μl / 50 μg in 100 μl tris-NaCl pH8
State:
Purified
Specificity
Specificity:
Human IgE (heat labile epitope in N-terminal of CH3 domain).
Cross–reactivity: does not react with human IgG, IgA, IgM.
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