Product Description for Human IgE
Product is tested for Western blot / Immunoblot, Functional assay, Flow Cytometry.
Properties for Human IgE
|Product Category||Secondary Antibodies|
|Synonyms||Immunoglobulin E human|
|Applications||F, FN, WB|
|Shipping to||Germany, USA/Canada|
|PDF datasheet||View Datasheet|
|Isotype control||AM03095PU-N, SM10P (for use in human samples)|
|Application||FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
|Background||Cell surface receptors for the Fc fragments of IgE (FcR) have been identified on a variety of cell types from hematopoietic origin. The low affinity receptor for IgE (CD23) has been described as a B cell-activation marker. IgE-binding factors have been shown to play a major role in the regulation of IgE production. Basophils and mast cells express FcR1, which bind IgE with high affinity and the reaction of allergen with cell-bound IgE induces the release of a variety of pharmacologically active mediators that are responsible for the clinical manifestations of allergic disorders. mAb27 was generated from a mouse immunization program aiming at producing mAb specific for human IgE-Fc in order to map the sites on IgE interacting with receptors on target cells. (Chrétien I. et al, J. Immunol, 1988, (9) 141, pp3128-34).|
|Storage||-20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.