DDX0290P-100 Human IgE antibody

Search for all "Human IgE"

0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Human IgE

Presentation: Purified
Product is tested for Western blot / Immunoblot, Functional assay, Flow Cytometry.

Properties for Human IgE

Product Category Secondary Antibodies
Quantity 0.1 mg
Synonyms Immunoglobulin E human
Presentation Purified
Reactivity Hu
Applications F, FN, WB
Clonality Monoclonal
Clone I27/mAb27
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Purified human IgE (preincubated with mouse serum anti human IgG)
Application FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). 
Usage recommendation
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Cell surface receptors for the Fc fragments of IgE (FcR) have been identified on a variety of cell types from hematopoietic origin. The low affinity receptor for IgE (CD23) has been described as a B cell-activation marker. IgE-binding factors have been shown to play a major role in the regulation of IgE production. Basophils and mast cells express FcR1, which bind IgE with high affinity and the reaction of allergen with cell-bound IgE induces the release of a variety of pharmacologically active mediators that are responsible for the clinical manifestations of allergic disorders. mAb27 was generated from a mouse immunization program aiming at producing mAb specific for human IgE-Fc in order to map the sites on IgE interacting with receptors on target cells. (Chrétien I. et al, J. Immunol, 1988, (9) 141, pp3128-34).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Chrétien I, Helm BA, Marsh PJ, Padlan EA, Wijdenes J, Banchereau J. A monoclonal anti-IgE antibody against an epitope (amino acids 367-376) in the CH3 domain inhibits IgE binding to the low affinity IgE receptor (CD23). J Immunol. 1988 Nov 1;141(9):3128-34. PubMed PMID: 2459242.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Buffer System:
100 μg in 200 μl / 50 μg in 100 μl tris-NaCl pH8
Human IgE (heat labile epitope in N-terminal of CH3 domain).
Cross–reactivity: does not react with human IgG, IgA, IgM.
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