AM31707AF-N Human IgG, IgA, IgM, IgD, IgE antibody

Search for all "Human IgG, IgA, IgM, IgD, IgE"

5 vials / €800.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Human IgG, IgA, IgM, IgD, IgE

Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Human IgG, IgA, IgM, IgD, IgE

Product Category Secondary Antibodies
Quantity 5 vials
Synonyms Human Immunoglobulin A, Human Immunoglobulin D, Human Immunoglobulin E, Human Immunoglobulin G, Human Immunoglobulin M
Presentation Ascites
Reactivity Hu
Applications C, E, ICC/IF, IP, R, WB
Clonality Monoclonal
Clone NI 335 and NI 343, NI 69 and NI 184, NI 179, NI 158, NI 307
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Highly purified monoclonal IgG subclasses isolated from pooled human serum.
5 Vials with 0.5 ml lyophilized Ascites each.
Add. information This Set contains 5 different Monoclonal Antibodies:
Cat.-No AM20258AF-N  Human IgG  (clone NI 335 and NI 343)
Cat.-No AM20259AF-N  Human IgM  (clone NI 179)
Cat.-No AM20249AF-N  Human IgA  (clone NI 69 (A89-034) and NI 184 (A89-035))
Cat.-No AM20250AF-N  Human IgD  (clone NI 158)
Cat.-No AM20251AF-N  Human IgE  (clone NI 307)
Application Can be used to identify the presence of immunoglobulin classes in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radioimmunoassay, ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
Recommended Working Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1000 Upwards.
Western blotting: 1/2000 Upwards.
Concentration 1.0 mg/ml (after reconstitution)
General Readings Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Delipidated, heat inactivated, lyophilized stable ascites.
Restore with 0.5 ml sterile distilled water.
The reactivity of the antibody is restricted to a isotype specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.
Cross-reactivity: The antibodies do not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. These antisera have not been tested for cross-reactivity with other species.
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