AM21029SU-N Human IgG1, IgG2, IgG3, IgG4 antibody

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Product Description for Human IgG1, IgG2, IgG3, IgG4

Presentation: Ascites
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Human IgG1, IgG2, IgG3, IgG4

Product Category Secondary Antibodies
Quantity 5x0.5 mg
Synonyms Human Immunoglobulin G1, Human Immunoglobulin G2, Human Immunoglobulin G3, Human Immunoglobulin G4
Presentation Ascites
Reactivity Hu
Applications Dot, E, ICC/IF, ID, IP, R, WB
Clonality Monoclonal
Clone NI132+NI6014+NI25-1+NI330+NI315
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Highly purified monoclonal IgG subclasses isolated from pooled Human serum.
Application To identify the presence of IgG subclasses in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG, subclasses and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/100-1/500.
ELISA: from 1/1,000 upwards.
Western blot: 1/2,000-1/10,000.
Background This Mouse ascites against Human IgG1 IgG2 IgG3 IgG4 (subclass specific) contains 5 x 0.5 mg Monoclonal Antibodies:
AM20252AF-N: clone NI 132 (HP 6186)
AM20253AF-N: clone NI 6014 (HP 6014)
AM20254AF-N: clone NI 25-1 (HP 6207)
AM20255AF-N: clone NI 330 (HP 6050)
AM20257AF-N: clone NI 315 (HP 6206)
Concentration 1.0 mg/ml
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Lyophilized (Delipidated, heat inactivated) stable ascites without preservatives or foreign proteins
Restore by adding 0.5 ml sterile distilled water.
Dilutions may be prepared by adding phosphate buffered saline PBS, pH 7.2 .
The reactivity of the antibody is restricted to a subclass specific determinant as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic immunoglobulins.gi
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