Product Description for Human IgG1+ IgG2 + IgG3 + IgG4
Product is tested for Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.
Properties for Human IgG1+ IgG2 + IgG3 + IgG4
|Product Category||Secondary Antibodies|
|Synonyms||Human Immunoglobulin G1, Human Immunoglobulin G2, Human Immunoglobulin G3, Human Immunoglobulin G4|
|Applications||Dot, E, ICC/IF, ID, IP, R, WB|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Polyclonal Antibodies (de)|
|Application||In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify and measure the concentration of IgG subclasses in serum or other body fluids. Human IgG constitutes about 75% of the total Immunoglobulins in the serum of normal adults. It consists of four subclasses which are distinguished in isotypic heterogeneity of the gamma chains, and in physicochemical, biological and effector properties. The term “subclass” designates the high degree of homology in amino acid sequences and antigenic structure. In normal adult serum, the approximate percentage composition of IgG with respect to its subclasses is: IgG1: 65%, IgG2: 23%, IgG3: 8% and IgG4: 4%. All four subclasses appear in both isotypes of the light chain. Normal serum thus contains eight different molecular forms of IgG, the absolute and relative distribution being genetically controlled and resulting from individual responses to the antigenicity of the environment. Antigenic determinants carrying isotypic subclass specificity can be situated on any of the three structurally very similar sub-regions o the gamma chains, known as “domains”. In IgG, some antigenic determinants are shared by two or three subclasses, but not by all four. Consequently, antibodies to such determinants will not be strictly subclass-specific. For the identification of subclasses of human IgG in cells and tissues by immunofluorescence or immunocytochemical peroxidase techniques, or in body fluids by ELISA and Western blotting type procedures, monoclonal antibody reagents to the individual subclasses are available.
Directions for use:
Because of their characteristics and those of the subclass-specific antigenic determinants, the use of these antisera requires special precautions. Their specificity and reactivity depend largely on the conditions of the test system in which they are applied. The performance of polyclonal subclass-specific antisera is guaranteed when they are used for the purposes described in RWP 5E/88, applying the special immunoprecipitation arrangement recommended for these purposes. In immunoelectrophoresis use 2 μl 1/8, 1/16 and 1/32 dilutions of pathological serum or equivalent against 60 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. We also makes available a human standard serum with assigned values of the subclasses of IgG.
|Storage||Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.