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AM01158AP-N Human IgG4 (pFC) antibody

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1 ml / €280.00
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AM01158AP-N

Product Description for Human IgG4

Properties: (pFC)
Presentation: AP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Human IgG4

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Human Immunoglobulin G4
Presentation AP
Reactivity Hu
Applications C, E, WB
Clonality Monoclonal
Clone HP6023 (also known as HO6023)
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Human IgG4 myeloma protein
Isotype control AM03097PU-N
Application ELISA: 1/1000-1/2000 (See also Ref.2-9). 
FLISA. 
Applications Reported in Literature: 
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Product Citations
Originator or purchased from resellers:
  1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984 Fall;3(3):263-75. PubMed PMID: 6209201.
  2. Arnold ML, Dechant M, Doxiadis II, Spriewald BM. Prevalence and specificity of immunoglobulin G and immunoglobulin A non-complement-binding anti-HLA alloantibodies in retransplant candidates. Tissue Antigens. 2008 Jul;72(1):60-6. doi: 10.1111/j.1399-0039.2008.01067.x. PubMed PMID: 18588575.
  3. Bogdanos D, Pusl T, Rust C, Vergani D, Beuers U. Primary biliary cirrhosis following Lactobacillus vaccination for recurrent vaginitis. J Hepatol. 2008 Sep;49(3):466-73. doi: 10.1016/j.jhep.2008.05.022. Epub 2008 Jun 25. PubMed PMID: 18644655.
  4. Forthal DN, Landucci G, Ding H, Kappes JC, Wang A, Thung I, et al. IgG2 inhibits HIV-1 internalization by monocytes, and IgG subclass binding is affected by gp120 glycosylation. AIDS. 2011 Nov 13;25(17):2099-104. doi: 10.1097/QAD.0b013e32834b64bd. PubMed PMID: 21832933.
  5. Jia XY, Hu SY, Chen JL, Qu Z, Liu G, Cui Z, et al. The clinical and immunological features of patients with combined anti-glomerular basement membrane disease and membranous nephropathy. Kidney Int. 2014 Apr;85(4):945-52. doi: 10.1038/ki.2013.364. Epub 2013 Sep 18. PubMed PMID: 24048374.
  6. Xu J, Wang L, Deng C, Hu X, Li R, Chen T, et al. The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection. Parasitol Res. 2014 Jan;113(1):149-55. doi: 10.1007/s00436-013-3637-3. Epub 2013 Oct 30. PubMed PMID: 24169809.
  7. Zhang J, Zhao L, Gao Y, Liu M, Li T, Huang Y, et al. A classification of Hashimoto's thyroiditis based on immunohistochemistry for IgG4 and IgG. Thyroid. 2014 Feb;24(2):364-70. doi: 10.1089/thy.2013.0211. Epub 2013 Nov 14. PubMed PMID: 23992023.
  8. Westra J, van Assen S, Wilting KR, Land J, Horst G, de Haan A, et al. Rituximab impairs immunoglobulin (Ig)M and IgG (subclass) responses after influenza vaccination in rheumatoid arthritis patients. Clin Exp Immunol. 2014 Oct;178(1):40-7. doi: 10.1111/cei.12390. PubMed PMID: 24889761. (Free PMC Article available, 3 images available)
  9. Seitz-Polski B, Payré C, Ambrosetti D, Albano L, Cassuto-Viguier E, Berguignat M, et al. Prediction of membranous nephropathy recurrence after transplantation by monitoring of anti-PLA2R1 (M-type phospholipase A2 receptor) autoantibodies: a case series of 15 patients. Nephrol Dial Transplant. 2014 Dec;29(12):2334-42. doi: 10.1093/ndt/gfu252. Epub 2014 Jul 25. PubMed PMID: 25063424.
  10. Detlefsen S, Bräsen JH, Zamboni G, Capelli P, Klöppel G. Deposition of complement C3c, immunoglobulin (Ig)G4 and IgG at the basement membrane of pancreatic ducts and acini in autoimmune pancreatitis. Histopathology. 2010 Dec;57(6):825-35. doi: 10.1111/j.1365-2559.2010.03717.x. PubMed PMID: 21166697.
  11. Qu Z, Liu G, Li J, Wu LH, Tan Y, Zheng X, et al. Absence of glomerular IgG4 deposition in patients with membranous nephropathy may indicate malignancy. Nephrol Dial Transplant. 2012 May;27(5):1931-7. doi: 10.1093/ndt/gfr534. Epub 2011 Oct 5. PubMed PMID: 21976738.
  12. de Bruin A, Caldelari R, Williamson L, Suter MM, Hunziker T, Wyder M, et al. Plakoglobin-dependent disruption of the desmosomal plaque in pemphigus vulgaris. Exp Dermatol. 2007 Jun;16(6):468-75. PubMed PMID: 17518986.
  13. Müller E, Kernland K, Caldelari R, Wyder M, Balmer V, Hunziker T. Unusual pemphigus phenotype in the presence of a Dsg1 and Dsg3 autoantibody profile. J Invest Dermatol. 2002 Mar;118(3):551-5. PubMed PMID: 11874500.
  14. Bogdanos DP, Pares A, Baum H, Caballeria L, Rigopoulou EI, Ma Y, et al. Disease-specific cross-reactivity between mimicking peptides of heat shock protein of Mycobacterium gordonae and dominant epitope of E2 subunit of pyruvate dehydrogenase is common in Spanish but not British patients with primary biliary cirrhosis. J Autoimmun. 2004 Jun;22(4):353-62. PubMed PMID: 15120760.
  15. Keen J, Serghides L, Ayi K, Patel SN, Ayisi J, van Eijk A, et al. HIV impairs opsonic phagocytic clearance of pregnancy-associated malaria parasites. PLoS Med. 2007 May;4(5):e181. PubMed PMID: 17535103. (Free PMC Article available)
  16. Parcina M, Miranda-Garcia MA, Durlanik S, Ziegler S, Over B, Georg P, et al. Pathogen-triggered activation of plasmacytoid dendritic cells induces IL-10-producing B cells in response to Staphylococcus aureus. J Immunol. 2013 Feb 15;190(4):1591-602. doi: 10.4049/jimmunol.1201222. Epub 2013 Jan 16. PubMed PMID: 23325892.
  17. Savilahti EM, Rantanen V, Lin JS, Karinen S, Saarinen KM, Goldis M, et al. Early recovery from cow's milk allergy is associated with decreasing IgE and increasing IgG4 binding to cow's milk epitopes. J Allergy Clin Immunol. 2010 Jun;125(6):1315-1321.e9. doi: 10.1016/j.jaci.2010.03.025. Epub 2010 May 11. PubMed PMID: 20462631. (Free PMC Article available, 4 images available)
  18. Johansson SE, Hejdeman B, Hinkula J, Johansson MH, Romagné F, Wahren B, et al. NK cell activation by KIR-binding antibody 1-7F9 and response to HIV-infected autologous cells in viremic and controller HIV-infected patients. Clin Immunol. 2010 Feb;134(2):158-68. doi: 10.1016/j.clim.2009.10.001. Epub 2009 Nov 1. PubMed PMID: 19880352.
  19. Gao B, Moore C, Porcheray F, Rong C, Abidoglu C, DeVito J, et al. Pretransplant IgG reactivity to apoptotic cells correlates with late kidney allograft loss. Am J Transplant. 2014 Jul;14(7):1581-91. doi: 10.1111/ajt.12763. Epub 2014 Jun 16. PubMed PMID: 24935695. (Free PMC Article available, 6 images available)
  20. Lowe D, Higgins R, Zehnder D, Briggs DC. Significant IgG subclass heterogeneity in HLA-specific antibodies: Implications for pathogenicity, prognosis, and the rejection response. Hum Immunol. 2013 May;74(5):666-72. doi: 10.1016/j.humimm.2013.01.008. Epub 2013 Jan 29. PubMed PMID: 23369861.
  21. Arnold ML, Ntokou IS, Doxiadis II, Spriewald BM, Boletis JN, Iniotaki AG. Donor-specific HLA antibodies: evaluating the risk for graft loss in renal transplant recipients with isotype switch from complement fixing IgG1/IgG3 to noncomplement fixing IgG2/IgG4 anti-HLA alloantibodies. Transpl Int. 2014 Mar;27(3):253-61. doi: 10.1111/tri.12206. Epub 2013 Oct 31. PubMed PMID: 24118579.
  22. Schaub S, Hönger G, Koller MT, Liwski R, Amico P. Determinants of C1q binding in the single antigen bead assay. Transplantation. 2014 Aug 27;98(4):387-93. doi: 10.1097/TP.0000000000000203. PubMed PMID: 24918611.
General Readings
  1. Mutapi F, Mduluza T, Ndhlovu PD. The effect of treatment on the age-antibody relationship in children infected with Schistosoma mansoni and Schistosoma haematobium. Mem Inst Oswaldo Cruz. 2002;97 Suppl 1:173-80. PubMed PMID: 12426616.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase
AP
Buffer System:
50 mM Tris/ 1 mM MgCl2, 50% Glycerol, pH 8.2
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This Monoclonal antibody recognizes the pFc' region of Human IgG4. 
No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA. Cross reactivity with immunoglobulins from other species has not been tested.
Species:
Human.
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