AM33324PU-T Human IgM (heavy chain) antibody

Search for all "Human IgM"

20 µg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Human IgM

Properties: (heavy chain)
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Human IgM

Product Category Secondary Antibodies
Quantity 20 µg
Synonyms Human Immunoglobulin M
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone IM373
Host Mouse
Isotype IgG2b
Molecular weight 50-75kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Recombinant heavy chain of Human IgM.
Isotype control AM03110PU-N, SM12P
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Concentration 0.2 mg/ml
General Readings
  1. Baryshnikov AIu. [Monoclonal antibodies of the ICO series against differentiation antigens of human lymphocytes]. Gematol Transfuziol. 1990 Aug;35(8):4-7. PubMed PMID: 2253863.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Recognizes a protein of 75kDa, identified as mu heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
This Monoclonal Antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Cellular Localization: Cytoplasm, Cell Surface and Secreted.
Species:
Human.
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