AM50119PU-T Human IgM (heavy chain) antibody

Search for all "Human IgM"

20 µg / €160.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Human IgM

Properties: (heavy chain)
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Human IgM

Product Category Secondary Antibodies
Quantity 20 µg
Synonyms Human Immunoglobulin M
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone SPM557
Host Mouse
Isotype IgG1
Molecular weight 75kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Recombinant heavy chain of human IgM.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Concentration 0.2 mg/ml
General Readings
  1. Maruyama S, Kubagawa H, Cooper MD. Activation of human B cells and inhibition of their terminal differentiation by monoclonal anti-mu antibodies. J Immunol. 1985 Jul;135(1):192-9. PubMed PMID: 3923101.
  2. Rudich SM, Winchester R, Mongini PK. Human B cell activation. Evidence for diverse signals provided by various monoclonal anti-IgM antibodies. J Exp Med. 1985 Oct 1;162(4):1236-55. PubMed PMID: 2413155. (Free PMC Article available)
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G Chromatography
Buffer System:
10mM PBS
Preservatives:
0.05% Sodium Azide
Stabilizers:
0.05% BSA
State:
Liquid purified IgG fraction from Bioreactor Concentrate
Purified
Specificity
Specificity:
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Cellular Localization: Cytoplasm, Cell Surface and Secreted.
Species:
Human.
  • LinkedIn