AP31435FC-N Monkey IgA + IgG + IgM (H+L chain) antibody

Search for all "Monkey IgA + IgG + IgM"

1 ml / €340.00
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AP31435FC-N

Product Description for Monkey IgA + IgG + IgM

Properties: (H+L chain)
Presentation: FITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Monkey IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Monkey IgA, Monkey IgG, Monkey IgM
Presentation FITC
Reactivity Mky
Applications C, E, ICC/IF, IP
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Concentration 10 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate isomer 1
FITC
Absorption emission:
492nm / 515nm
Molar radio:
1,6
Buffer System:
PBS, pH 7.2
No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
State:
Lyophilised hyperimmune Ig fraction
Reconstitution:
Restore with 1 ml sterile di stilled water
Specificity
Specificity:
The reactivity of the antiserum is directed to the major isotypes of the monkey immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for chimpanzee and man.
Species:
Monkey.
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