AP31436HR-N Monkey IgA + IgG + IgM (Fc specific) antibody

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1 ml / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP31436HR-N

Product Description for Monkey IgA + IgG + IgM

Properties: (Fc specific)
Presentation: HRP
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Monkey IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Monkey IgA, Monkey IgG, Monkey IgM
Presentation HRP
Reactivity Mky
Applications C, Dot, E, ICC/IF, ID, IP, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted monkey serum fractions and IgG/Fab as required to eliminate antibodies reacting with the common Fab portion of immunoglobulins or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000.
Concentration 10 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Molar radio:
1,7
Buffer System:
PBS, pH 7.2
No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
State:
Lyophilised hyperimmune Ig fraction
Reconstitution:
Restore with 1 ml sterile distilled water
Specificity
Specificity:
The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the monkey immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for chimpanzee and man.
Species:
Monkey.
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