AP31447BT-N Monkey IgA + IgG + IgM (H+L chain) antibody

Search for all "Monkey IgA + IgG + IgM"

1 ml / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

AP31447BT-N

Product Description for Monkey IgA + IgG + IgM

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Monkey IgA + IgG + IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Monkey IgA, Monkey IgG, Monkey IgM
Presentation Biotin
Reactivity Mky
Applications C, Dot, E, ICC/IF, ID, IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Highly purified IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Can be usde in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/20000.
Concentration 10 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Marker: N-Hydroxysuccinimidobiotin.Conjugation procedure: Conjugation is carried out using a proprietary technique for the binding to biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. No foreign protein has been added
Biotin
Molar radio:
4,0
Purification:
DEAE-column Chromatography
Buffer System:
PBS, pH 7.2
No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
State:
Lyophilised hyperimmune Ig fraction
Reconstitution:
Restore with 1 ml sterile distilled water
Specificity
Specificity:
The defined antibody specificity is directed to the major isotypes of the monkey immunoglobulin system and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony).
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for chimpanzee and man.
Species:
Monkey.
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