discontinued

NB120-5887 Mouse IgG - F(ab)2 Fragment antibody

Search for all "Mouse IgG - F(ab)2 Fragment"

0.5 mg / €360.00

NB120-5887

Product Description for Mouse IgG - F(ab)2 Fragment

Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Mouse IgG - F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.5 mg
Presentation HRP
Reactivity Ms
Applications E, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG F(ab)2 fragment
Add. information This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunofluorescence, immunohistochemistry, Western Blot, This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Optimal dilutions/concentrations should be determined by the end user., ELISA 1/8000 - 1/32000, immunohistochemistry , immunofluorescence , Western Blot
Concentration 0.5 mg/ml
General Readings General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
HRP
Purification:
Affinity purified
Buffer System:
0.01% Gentamicin Sulfate
Purity:
Affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, H????
Species:
Mouse
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