Product Description for Mouse IgG (H&L) F(ab)2 Fragment
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Properties for Mouse IgG (H&L) F(ab)2 Fragment
| Product Category | Secondary Antibodies |
|---|---|
| Quantity | 0.1 mg |
| Presentation | Biotin |
| Reactivity | Ms |
| Applications | E, P, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
|
|---|---|
| Add. information | Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/7000 - 1/70000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Application | ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/7000 - 1/70000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, immunohistochemistry, immunofluorescence, Western Blot |
| Concentration | 0.5 mg/ml |
| General Readings | General / background references:Bayer EA & Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990). PubMed PMID: 2388567 |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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