discontinued

NB120-5761 Mouse IgG (H&L) F(ab)2 Fragment antibody

Search for all "Mouse IgG (H&L) F(ab)2 Fragment"

0.1 mg / €250.00

NB120-5761

Product Description for Mouse IgG (H&L) F(ab)2 Fragment

Presentation: Biotin
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Mouse IgG (H&L) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.1 mg
Presentation Biotin
Reactivity Ms
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/7000 - 1/70000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/7000 - 1/70000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA, immunohistochemistry, immunofluorescence, Western Blot
Concentration 0.5 mg/ml
General Readings General / background references:Bayer EA & Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990). PubMed PMID: 2388567
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
Biotin
Purification:
Immunogen affinity purified
Buffer System:
0.01% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Species:
Mouse
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