discontinued

NB120-5763 Mouse IgG (H&L) F(ab)2 Fragment antibody

Search for all "Mouse IgG (H&L) F(ab)2 Fragment"

0.25 mg / €250.00

NB120-5763

Product Description for Mouse IgG (H&L) F(ab)2 Fragment

Presentation: AP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Mouse IgG (H&L) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation AP
Reactivity Ms
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/5000 - 1/25000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 m g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/5000 - 1/25000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 m g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA 1:4,000 - 1:20,000, immunohistochemistry 1:200 - 1:1,000, Western Blot 1:1,000 - 1:5,000
Concentration 0.7 mg/ml
General Readings General / background references:Avrameas S & Ternynck T Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175-9 (1971). PubMed PMID: 5150003
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Label:
Alkaline Phosphatase
AP
Purification:
Immunogen affinity purified
Buffer System:
0.01% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Species:
Mouse
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