Product Description for Mouse IgG (H&L) Fab Fragment
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Properties for Mouse IgG (H&L) Fab Fragment
| Product Category | Secondary Antibodies |
|---|---|
| Quantity | 0.5 mg |
| Presentation | Biotin |
| Reactivity | Ms |
| Applications | E, P, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/30000 - 1/140000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/30000 to 1/140000 of the reconstitution concentration is suggested for this product. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Application | ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/30000 - 1/140000. IHC: Use at an assay dependent dilution. IM: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/30000 to 1/140000 of the reconstitution concentration is suggested for this product. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., ELISA 1:200000, immunohistochemistry 1:1000-1:5000, immunofluorescence , Western Blot 1:2000-1:10000, |
| Concentration | 1 mg/ml |
| General Readings | General / background references:Bayer EA & Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990). PubMed PMID: 2388567 |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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