discontinued

NB120-6007 Mouse IgG + IgM + IgA (H&L) F(ab)2 Fragment antibody

Search for all "Mouse IgG + IgM + IgA (H&L) F(ab)2 Fragment"

0.25 mg / €220.00

NB120-6007

Product Description for Mouse IgG + IgM + IgA (H&L) F(ab)2 Fragment

Presentation: AP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Mouse IgG + IgM + IgA (H&L) F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation AP
Reactivity Ms
Applications E, P, WB
Clonality Polyclonal
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG, IgA and IgM whole molecules
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/1000 - 1/3000. IHC: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/3000 of the reconstitution concentration is suggested for this productSuitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Application ELISA, immunohistochemistry, Western Blot, ELISA, Immunohistochemistry and Western Blot: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1000 to 1/3000 of the reconstitution concentration is suggested for this product suitable for other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. , ELISA 1:1,000 - 1:5,000, immunohistochemistry 1:200 - 1:1,000, Western Blot 1:500 - 1:2,000
Concentration 1 mg/ml
General Readings General / background references:Avrameas S & Ternynck T Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry 8:1175-9 (1971). PubMed PMID: 5150003
Storage Store at 4C. Do not freeze.
Format
Label:
Alkaline Phosphatase
AP
Purification:
Affinity purified
Buffer System:
0.01% Sodium Azide
Purity:
Affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase and anti-Goat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Human Serum Proteins. This reagent is suitable for the detection ????
Species:
Mouse
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