R1469B Mouse IgG + IgM (H+L chain), adsorbed antibody

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1 mg / €250.00
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R1469B

Product Description for Mouse IgG + IgM

Properties: (H+L chain), adsorbed
Presentation: Biotin

Properties for Mouse IgG + IgM

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Mouse Immunoglobulin G
Presentation Biotin
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG and IgM whole molecules
Application Suitable for Immunoblotting (Western Blot, 1:2,000-1:10,000), ELISA (1:20,000-1:100,000), Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Storage Store the antibody at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Centrifuge product if not completely clear after standing at room
temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per bbb IgG molecule.
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified IgG fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
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