R1469HRP Mouse IgG + IgM (H+L chain), adsorbed antibody

Search for all "Mouse IgG + IgM"

1 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Mouse IgG + IgM

Properties: (H+L chain), adsorbed
Presentation: HRP

Properties for Mouse IgG + IgM

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Mouse Immunoglobulin G
Presentation HRP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Mouse IgG and IgM whole molecules.
Application Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/40,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Caution Do Not Use Sodium Azide as preservative.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Horseradish Peroxidase
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
Lyophilized purified IgG fraction.
Restore with 1.0 ml of deionized water (or equivalent).
This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Mouse IgG and Mouse IgM.
No reaction was observed against Bovine, Horse or Human serum proteins.
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