R1253AP Mouse IgG (H+L chain) antibody

Search for all "Mouse IgG"

1 mg / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1253AP

Product Description for Mouse IgG

Properties: (H+L chain)
Presentation: AP
Product is tested for Western blot / Immunoblot, Frozen Sections, Enzyme Immunoassay.

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Mouse Immunoglobulin G
Presentation AP
Applications C, E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule
Application Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Concentration 1.0 mg/ml (by UV Absorbance at 280 nm)
General Readings Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Dilute only prior to immediate use.
Freezing Alkaline Phosphatase conjugates will result in a substantial loss of enzymatic activity.

Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, containing 10 mg/ml BSA (Ig and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Purity:
and
State:
Liquid purified Ig fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum.
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