R1253HRP Mouse IgG (H+L chain) antibody

Search for all "Mouse IgG"

2 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Mouse IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Frozen Sections, Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Mouse Immunoglobulin G
Presentation HRP
Applications C, E, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 40,000 Da
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Caution Do Not Add Sodium Azide.
Concentration 2.0 mg/ml (by UV absorbance at 280nm)
General Readings
  1. Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation).
  2. Sone H, Kagawa Y. Pancreatic beta cell senescence contributes to the pathogenesis of type 2 diabetes in high-fat diet-induced diabetic mice. Diabetologia. 2005 Jan;48(1):58-67. Epub 2004 Dec 29. PubMed PMID: 15624098.
Storage Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot and freeze at -20°C or below.
Avoid cycles of freezing and thawing! Dilute only prior to immediate use!

Format
Label:
Horseradish Peroxidase
Conjugation Chemistry: Periodate
HRP
Purification:
Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Gentamicin Sulfate
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
Purity:
and
State:
Lyophilized purified Ig fraction
Reconstitution:
Reconstitute with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
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