R1403R Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

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0.5 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1403R

Product Description for Mouse IgG

Properties: (H+L chain), F(ab)2 Fragment, adsorbed
Presentation: PE
Product is tested for Flow Cytometry.

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Mouse Immunoglobulin G
Presentation PE
Applications F
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule
Add. information OD566nm/OD280nm: 2.8
Application Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Concentration 0.5 mg/ml (determined by absorbance = 8.2 at 565 nm for a 0.1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed
PE
Absorption emission:
488 nm / 575 nm
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
State:
Lyophilized purified F(ab')2 Fragment
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent)
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
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