R1405R Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

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0.5 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1405R

Product Description for Mouse IgG

Properties: (H+L chain), F(ab)2 Fragment, adsorbed
Presentation: PE

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Mouse Immunoglobulin G
Presentation PE
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Add. information OD566nm/OD280nm: 2.7
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis (1/100-1/250) as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 mg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Concentration Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
Storage This antibody is stable at 2-8°C as an undiluted liquid.
DO NOT FREEZE!
Restore with 1.0 ml of deionized water (or equivalent).
Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed
PE
Absorption emission:
490 nm, 545 nm and 565 nm / 580 nm
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized F(ab')2 fragments.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit or Swine Serum Proteins.
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