R1455R Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody

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0.5 mg / €380.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1455R

Product Description for Mouse IgG

Properties: (H+L chain), F(ab)2 Fragment, adsorbed
Presentation: PE

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 0.5 mg
Synonyms Mouse Immunoglobulin G
Presentation PE
Clonality Polyclonal
Host Donkey
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG whole molecule.
Application Suitable for Immunomicroscopy and Flow Cytometry or FACS as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry: the maximum amount of reagent required to stain 1x10e6 cells is approx. 1.0 µg of antibody conjugate.
As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Background Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10e11 variants.
Concentration 0.5 mg/ml (determined by absorbance = 82.0 at 565nm).
Storage Store vial at 2-8°C prior to opening. Restore with deionized water or equivalent.
This product is stable at 2-8°C as an undiluted liquid.
Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute the antibody only prior to immediate use.
Shelf life: One year from despatch.
Format
Label:
R-Phycoerythrin ( (Molecular Weight 240,000 daltons) from seaweed
PE
Absorption emission:
490, 545, 565 nm / 580 nm
Purification:
Immunoaffinity Chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA as stabilizer and 0.02% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified IgG fraction.
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Mouse IgG and Mouse Serum.
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, Rat or Sheep serum proteins.
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