discontinued

R1457C5 Mouse IgG (H+L chain), adsorbed antibody

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R1457C5

Product Description for Mouse IgG

Properties: (H+L chain), adsorbed
Presentation: Cy5

Properties for Mouse IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Mouse Immunoglobulin G
Presentation Cy5
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Mouse IgG, whole molecule
Application
This product is designed for Immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.  
Recommended Dilurtions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Concentration 1.09 mg/ml (by UV absorbance at 280 nm)
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
(Molecular Weight 975 daltons)
Cy5
Absorption emission:
650 nm / 667 nm
Molar radio:
16.2 moles Cy5™ per mole of Goat IgG
Purification:
Immunoaffinity Chromatography
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% Sodium Azide
Stabilizers:
10 mg/ml BSA (IgG and Protease free)
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Species Reactivity
Species reactivity (tested):
Mouse.
Specificity
Specificity:
This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.  
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. 
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rat, Rabbit and Sheep Serum Proteins.
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