AP33463AP-N Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) antibody

Search for all "Mouse IgG1"

1 mg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Mouse IgG1

Properties: (Ads. to Hu, Bov, Rb Serum Proteins)
Presentation: AP
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay, Frozen Sections.

Properties for Mouse IgG1

Product Category Secondary Antibodies
Quantity 1 mg
Synonyms Mouse Immunoglobulin G1
Presentation AP
Reactivity Ms
Applications C, E, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Native Mouse IgG1 heavy chain Protein.
Application Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western Blot: 1/200-1/2,000. 
Immunohistochemistry: 1/200-1/1,000.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Alkaline Phosphatase (Calf Intestine)
Immunoaffinity Chromatography using Mouse IgG1 coupled to Agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
0.09% (w/v) Sodium Azide
10 mg/ml BSA (Immunoglobulin and Protease free)
Liquid (sterile filtered) purified IgG fraction
Aff - Purified
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins.
Specificity was confirmed by ELISA at less than 1% of target signal.
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