AP21492SU-N Mouse IgG2ab (subclass specific) antibody

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Product Description for Mouse IgG2ab

Properties: (subclass specific)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Mouse IgG2ab

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Mouse Immunoglobulin G2ab
Presentation Serum
Reactivity Ms
Applications ID, IP
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a and Igh-1b.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in mouse serum and other body fluids or to determine its concentration.
To prepare an immunoadsorbent for the purification of Mouse IgG2 from serum or plasma.
Recommended Dilutions:
Imunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less then 1/16 when tested against pooled normal mouse serum in agar block immunodiffusion titration.
Concentration Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Storage Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.
Lyophilized (Delipidated, heat inactivated) stable whole antiserum without preservatives or foreign proteins.
Restore by adding 1 ml sterile distilled water
The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b.
The antiserum contains antibodies to iso and allospecific determinants. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in Mouse serum, as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.
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