AP32992FC-N Mouse IgM (Fc specific) antibody

Search for all "Mouse IgM"

1 ml / €430.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Mouse IgM

Properties: (Fc specific)
Presentation: FITC
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Mouse IgM

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Mouse Immunoglobulin M
Presentation FITC
Reactivity Ms
Applications C, E, ICC/IF, ID, IP
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application ELISA.
Immunohistochemistry on Frozen Sections.
(In)direct immunofluorescence.
In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions1/20- 1/80.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Absorption emission:
492 nm / 515 nm
Molar radio:
Fluorochrome/IgG protein (F/P) ~ 1.3
Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt precipitation and purification of the IgG fraction by DEAE Chromatography
Buffer System:
PBS, pH 7.2
None, as it may interfere with the antibody activity.
Lyophilized hyperimmune IgG fraction
Restore with 1 ml sterile distilled water.
The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma and serum, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No reaction is obtained with purified IgA, IgG and IgG/Fab fragments.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.
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