Product Description for Mouse IgM
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Mouse IgM
|Product Category||Secondary Antibodies|
|Synonyms||Mouse Immunoglobulin G|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Polyclonal Antibodies (de)|
|Add. information||Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.|
Can be used: In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma.
This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/32 when tested against pooled normal mouse serum in agar-block immunodiffusion titration.
|Concentration||Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.