discontinued

NB120-5929 Mouse IgM mu - F(ab)2 Fragment antibody

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0.25 mg / €290.00

NB120-5929

Product Description for Mouse IgM mu - F(ab)2 Fragment

Presentation: Biotin
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Mouse IgM mu - F(ab)2 Fragment

Product Category Secondary Antibodies
Quantity 0.25 mg
Presentation Biotin
Reactivity Ms
Applications E, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Mouse IgM mu heavy chain
Add. information Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/20000 - 1/400000. FACS: Use at a dilution of 1/200 - 1/1000. IHC: Use at a dilution of 1/500 - 1/5000. IM: Use at an assay dependent dilution. WB: Use at a dilution of 1/20000 - 1/400000. This product has been assayed against 1.0 ug of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2?-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Application ELISA, flow cytometry / FACS analysis, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/20000 - 1/400000. FACS: Use at a dilution of 1/200 - 1/1000. IHC: Use at a dilution of 1/500 - 1/5000. IM: Use at an assay dependent dilution. WB: Use at a dilution of 1/20000 - 1/400000. This product has been assayed against 1.0 ug of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user., flow cytometry / FACS analysis , ELISA 1:10,000 - 1:50,000, immunohistochemistry 1:500 - 1:2,500, immunofluorescence , Western Blot 1:1,000 - 1:5,000
Concentration 1 mg/ml
General Readings General / background references:Bayer EA & Wilchek M Protein biotinylation. Methods Enzymol 184:138-60 (1990). PubMed PMID: 2388567
Storage Store at 4C. Do not freeze.
Format
Label:
Biotin
Purification:
Immunogen affinity purified
Buffer System:
0.01% Sodium Azide
Purity:
Immunogen affinity purified
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Species:
Mouse
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