Product Description for Mouse IgM mu - F(ab)2 Fragment
Presentation: HRP
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.
Properties for Mouse IgM mu - F(ab)2 Fragment
| Product Category | Secondary Antibodies |
|---|---|
| Quantity | 0.25 mg |
| Presentation | HRP |
| Reactivity | Ms |
| Applications | E, P, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Isotype | IgG |
| Shipping to | Not USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Novus Biologicals Inc. |
Datasheet Extract
| Immunogen |
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|---|---|
| Add. information | Dot: Use at a dilution of 1/5000 - 1/100000. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1/5000 - 1/100000. IHC: Use at a dilution of 1/500 - 1/5000. WB: Use at a dilution of 1/5000 - 1/100000. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Application | ELISA, immunofluorescence, immunohistochemistry, Western Blot, Dot: Use at a dilution of 1:5000 - 1:100000. Electron Microscopy: Use at an assay dependent dilution. ELISA: Use at a dilution of 1:5000 - 1:100000. IHC: Use at a dilution of 1:500 - 1:5000. WB: Use at a dilution of 1:5000 - 1:100000. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., ELISA 1:10,000 - 1:50,000, immunohistochemistry 1:500 - 1:5,000, immunofluorescence , Western Blot 1:2,000 - 1:10,000 |
| Concentration | 1 mg/ml |
| General Readings | General / background references:Farr AG & Nakane PK Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Methods 47:129-44 (1981). PubMed PMID: 6172519 |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
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