AP33087HR-N Porcine IgG (H+L chain) antibody

Search for all "Porcine IgG"

1 ml / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Porcine IgG

Properties: (H+L chain)
Presentation: HRP
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Dot blot, Enzyme Immunoassay.

Properties for Porcine IgG

Product Category Secondary Antibodies
Quantity 1 ml
Synonyms Pig IgG, Pig Immunoglobulin G
Presentation HRP
Reactivity Por
Applications Dot, E, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Add. information Adsorption:  Not required.
Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.
Application ELISA. 
Dot blot.
Immunohistochemistry Paraffin Sections.

Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Concentration 10.0 mg/ml
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2)
Molar radio:
Peroxidase/IgG ~1.7
Hyperimmune antisera with strong activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Buffer System:
PBS, pH 7.2
None, as it may interfere with the antibody activity.
Lyophilized purified hyperimmune IgG fraction
Restore with 1 ml sterile distilled water.
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in swine serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion with the following results: Bovine: +, Cat: +, Chicken: -, Dog: +, Duck: -, Goat: +, Guinea pig: +, Human: +, Horse: +, Mouse: +, Monkey: +, Pigeon: -, Rat: +, Sheep: +, Turkey: -. 
A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive assays.
  • LinkedIn