Product Description for Porcine IgM
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation.
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation.
Properties for Porcine IgM
| Product Category | Secondary Antibodies |
|---|---|
| Target Category | |
| Quantity | 0.2 mg |
| Synonyms | Pig Immunoglobulin M |
| Presentation | Aff - Purified |
| Reactivity | Por |
| Applications | C, E, IP |
| Clonality | Monoclonal |
| Clone | 206-2 |
| Host | Mouse |
| Isotype | IgG2b |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Monoclonal Antibodies (de) |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | ELISA: 0.1 µg/ml (1/4000). Immunoprecipitation. Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000). Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section). Does not react in Western Blot with reduced and denatured samples. |
| Background | IgM antibodies form a major proportion of the primary antibody response, whereas the secondary response consists almost entirely of IgG. IgMs are often referred to as macroglobulins because of their high molecular weight. They form a pentameric structure consisting of five subunits and a single J-chain. Each subunit contains the well-known pairs of heavy and light chains, with the particular feature of the heavy (µ) chain containing one variable and four constant regions. The molecular weight of intact IgM is approximately 900kDa. In polyacrylamide gel electrophoresis under denaturing conditions, the heavy chain migrates as a band of approximately 85kDa. |
| Concentration | 0.4 mg/ml IgG (after reconstitution) |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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