AM03216PU-N Porcine IgM antibody

Search for all "Porcine IgM"

0.2 mg / €390.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Product Description for Porcine IgM

Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation.

Properties for Porcine IgM

Product Category Secondary Antibodies
Target Category
Quantity 0.2 mg
Synonyms Pig Immunoglobulin M
Presentation Aff - Purified
Reactivity Por
Applications C, E, IP
Clonality Monoclonal
Clone 206-2
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Porcine IgM.
Application ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Background IgM antibodies form a major proportion of the primary antibody response, whereas the secondary response consists almost entirely of IgG. IgMs are often referred to as macroglobulins because of their high molecular weight. They form a pentameric structure consisting of five subunits and a single J-chain. Each subunit contains the well-known pairs of heavy and light chains, with the particular feature of the heavy (µ) chain containing one variable and four constant regions. The molecular weight of intact IgM is approximately 900kDa. In polyacrylamide gel electrophoresis under denaturing conditions, the heavy chain migrates as a band of approximately 85kDa.
Concentration 0.4 mg/ml IgG (after reconstitution)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2 containing 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as preservative
State:
Lyophilized purified IgG fraction
Aff - Purified
Reconstitution:
Restore with 0.5 ml distilled water to make stock solution.
Species Reactivity
Species reactivity (tested):
Porcine.Minimal Cross Reaction with Rat, Goat, Sheep, Donkey, Human (all < 0.1%) and Rabbit (0.1%).
Specificity
Specificity:
Monoclonal antibody AM03216PU-N (Clone 206-2) recognizes the Porcine Immunoglobulin M (IgM) antibody protein (Minimal reaction with IgG).
Tissue Sections: B-cells and macrophages are stained in Swine spleen.
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