Product Description for Porcine IgM
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Porcine IgM
|Product Category||Secondary Antibodies|
|Synonyms||Pig Immunoglobulin M|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Polyclonal Antibodies (de)|
|Add. information||Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.|
|Application||In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgM from serum or plasma.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Precipitin titre: 1/32 when tested against pooled normal Swine serum in agar-block immunodiffusion titration.
|Concentration||Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.