AP31795AG-N PrecipHen® (Chicken IgY) antibody

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2 ml / €450.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for PrecipHen® (Chicken IgY)

Presentation: Agarose
Product is tested for Immunoprecipitation.

Properties for PrecipHen® (Chicken IgY)

Product Category Secondary Antibodies
Quantity 2 ml
Synonyms Chicken Immunoglobulin Y
Presentation Agarose
Reactivity Chk
Applications IP
Clonality Polyclonal
Host Goat
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immune sera was collected from goats that were hyper-immunized with purified chicken IgY.
Production: Goat anti-Chicken IgY antibodies were affinity purified from the sera over a Chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated Agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50mM HEPES buffer (pH 7.6) with 5mM EDTA (TE buffer). Sodium Azide was then added to a final concentration of 0.02% (w/v).
Application Immunoprecipitation.
Concentration 2.0 ml bed volume in a total of 4.0 ml
Storage Store the antibody at 2-8°C in the dark.
Shelf life: one year from despatch.
Affinity Chromatography
Buffer System:
50mM HEPES, pH 7.6, 5mM EDTA containing 0.02% Sodium Azide as preservative.
Agarose beads in buffer
Binding Capacity: 1.0 mg of Chicken IgY per ml bed volume.
Quality Control: The capacity of PrecipHen® was determined by measuring the 2.5 mls of PrecipHen. One milliliter aliquots of chicken IgY (200 µg/ml in TE buffer) were applied to the column and fractions were collected. A similar column of agarose (non-NHS activated) was used for comprison. The concentration of IgY passing through each column was measured, and the total amount of IgY that bound to the column was calculated.
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